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The extracellular generation of free radicals in ischemic tissue may be harmful to the cell, and a radical scavenger may be beneficial by limiting the extent of the damage. We therefore investigated whether antioxidants could prevent or attenuate ischemia/reperfusion damage. Myocardial oxygen consumption and lactate production were assessed in the isolated perfused rat heart using microdialysis technique to evaluate the vulnerability of the tissue to ischemia. The role of free radicals was demonstrated in an in vitro study, where myocardial oxygen consumption and lactate production were evaluated in a cell culture medium. Catalase was added to the medium as an antioxidant. H(2)O(2) was used as a free radical inducer. Lactate production and oxygen consumption were measured by flow-injection analysis. In the in vivo model of reperfusion, creatine kinase (CK) release and infarct size were assessed. It was demonstrated that the free radical scavenger catalase could protect the myocardium from reperfusion-induced damage by preventing lactate production. Protection was also afforded by the antioxidant ethoxyquin (EQ), as assessed both in vitro and in vivo. In the presence of catalase or EQ, CK release was reduced and infarct size was decreased. In the absence of catalase, neither EQ nor superoxide dismutase (SOD), an alternative antioxidant, were able to afford protection of the myocardium. These results demonstrate that catalase, but not SOD, affords protection of the myocardium against ischemic/reperfusion injury, and suggest that this effect is related to the extracellular generation of radicals.Q:
Find the integral of a function with a complicated integrand
I have a function that is defined as
$f(t) = e^{ -t} \int_{ -\infty}^{\infty}\cos x e^{ -(\cos x + t)\cos x}dx$
I have the answer as $ \frac{\pi^3}{8}$ (See
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